How do you isolate stem cells from adipose tissue?

How do you isolate stem cells from adipose tissue?

ASCs can be isolated from adipose tissue by first washing the tissue sample extensively with phosphate-buffered saline (PBS) containing 5% Penicillin/Streptomycin (P/S).

Where is the nucleus of an adipocyte located and why?

The nucleus is round and, although eccentrically located, it is not in the periphery of the cell. The brown color comes from the large quantity of mitochondria.

What is found in the adipocyte?

Adipose, or fat, tissue is loose connective tissue composed of fat cells known as adipocytes. Adipocytes contain lipid droplets of stored triglycerides. These cells swell as they store fat and shrink when the fat is used for energy.19-Aug-2019

Is subcutaneous and adipose the same?

The adipose tissue beneath the skin is called subcutaneous adipose tissue (SAT), whereas the one lining internal organs is termed visceral adipose tissue (VAT). There are considerable anatomical differences in the distribution of two adipose tissues in the body.

Can you gain adipose tissue?

Dieting can shrink fat cells but not eliminate them, which is why people can gain weight back so quickly. The Yale team found that the activation of a nutrient signaling pathway called PI3-kinase/AKT-2 was necessary to produce fat precursor cells, which in turn produce adipose tissue.Mar 2, 2015

What is meant by adipocyte?

Definition of adipocyte : a specialized cell of adipose tissue that stores excess energy in the form of triglyceride droplets and secretes various substances (such as leptin) that play a role in various physiological processes (such as regulation of food intake or lipid metabolism) : fat cell.

What is human adipocyte?

Abstract. Adipose-derived stem cells are multipotent cells that can easily be extracted from adipose tissue, are capable of expansion in vitro, and have the capacity to differentiate into multiple cell lineages, which have the potential for use in regenerative medicine.

Are adipocytes derived from mesoderm?

Adipose tissue is believed to be mesodermal in origin. Mesoderm arises during gastrulation as the middle layer of tissue formed by cells migrating between the endoderm and ectoderm.

Are adipocytes skin cells?

Adipocytes are intimately associated with the dermal compartment of the skin, existing in a specialized dermal depot and displaying dynamic changes in size during tissue homeostasis. However, the roles of adipocytes in cutaneous biology and disease are not well understood.

What is stored in adipose cells?

Adipose (fat) cellsfat) cellsAdipocyte progenitor cells (APCs) provide the reservoir of regenerative cells to produce new adipocytes, although their identity in humans remains elusive. Using FACS analysis, gene expression profiling, and metabolic and proteomic analyses, we identified three APC subtypes in human white adipose tissues. › Identification of Metabolically Distinct Adipocyte Progenitor Cells in are specialized for the storage of energy in the form of triglycerides, but research in the last few decades has shown that fat cells also play a critical role in sensing and responding to changes in systemic energy balance.

Can you culture adipocytes?

Using MAAC, adipocytes can be cultured from lean and obese patients, different adipose depots, co-cultured with different cell types, and importantly, can be kept in culture for 2 weeks. Functional experiments can also be performed on MAAC including glucose uptake, lipogenesis, and lipolysis.

How do you grow adipose tissue?

Adipose tissue grows by two mechanisms: hyperplasia (cell number increase) and hypertrophy (cell size increase). Genetics and diet affect the relative contributions of these two mechanisms to the growth of adipose tissue in obesity.

How do you isolate adipocytes?

Incubate in shaking water bath for 20-30 minutes at 37C with vigorous shaking. Cells should completely disintegrate into individual cells, extend the time if necessary. Centrifuge at 500g (2200 RPM) for 10 minutes to separate floating adipocytes from pelleted SVF (pre-adipocytes and leukocytes).

What molecule is stored in an adipocyte?

About 80% of average white adipose tissue is lipid, and of that about 90% is made up of the six triglycerides: stearic, oleic, linoleic, palmitic, palmitoleic and myristic acid. Also stored are free fatty acids, cholesterol, mono- and di-glycerides.

What is the adipocyte function?

The Adipocyte as Functional Endocrine Cell The classical function of the adipocyte is as a calorie storage system accepting chemical energy in the form of glucose and fatty acid from the blood and converting these metabolites to TG for storage during fed conditions via lipogenesis.

Is adipocyte a cell?

adipose cell, also called adipocyte or fat cell, connective-tissue cell specialized to synthesize and contain large globules of fat.adipose cell, also called adipocyte or fat cell, connective-tissueconnective-tissueConnective tissue is one of the many basic types of animal tissue, along with epithelial tissue, muscle tissue, and nervous tissue. In embryology it develops from the mesoderm. Connective tissue is found in between other tissues everywhere in the body, including the nervous system. › wiki › Connective_tissueConnective tissue – Wikipedia cell specialized to synthesize and contain large globules of fat.

What makes adipocytes unique?

While many species have evolved cells capable of lipid storage, the adipocyte represents a unique specialized cell involved in fuel storage, endocrine, nervous and immune function. However, the adipocytes are not the only cell type in mammals that can accumulate lipid droplets.

Adipocyte Cell Line – an overview | ScienceDirect Topics

In a human adipocyte cell line, a low dose of the synthetic glucocorticoid, dexamethasone, has a synergistic effect with insulin in increasing lipogenesis yet decreases lipogenesis in the absence of insulin10 (see Table 4.1 ). This suggests that the effects of glucocorticoids may vary between the fed and fasted state.

LS14: a novel human adipocyte cell line that produces

In summary, we have established a novel human adipocyte cell line with many characteristics of primary adipocytes. The LS14 cells open up new avenues for research on human adipocyte biology and add to the repertoire of nonpituitary, PRL-producing cell lines.

Human Adipocytes | HAd | Fat Cells from Adipose Tissue

Mature Human Adipocytes (HAd) are differentiated from HPAd (Human Preadipocytes) through the use of Adipocyte Differentiation Medium.Cells are shipped 5 days after initiating differentiation. Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation.

| Human adipocyte cell lines. | Download Table

The PAZ6 cell line is reported to be a human adipocyte cell line that has properties of brown adipocytes, notably UCP1 expression (Guennoun et al. 2015; Kazantzis et al. 2012). It was obtained by

Adipocyte Cell Line – an overview | ScienceDirect Topics

In a human adipocyte cell line, a low dose of the synthetic glucocorticoid, dexamethasone, has a synergistic effect with insulin in increasing lipogenesis yet decreases lipogenesis in the absence of insulin10 (see Table 4.1 ). This suggests that the effects of glucocorticoids may vary between the fed and fasted state.

The human Adipocytes – The Human Protein Atlas

Transcriptome analysis shows that 63% (n=12562) of all human proteins (n=20090) are detected in adipocytes and 623 of these genes show an elevated expression in any adipocytes compared to other cell type groups. In-depth analysis of the elevated genes in adipocytes using scRNA-seq and antibody-based protein profiling allowed us to visualize the expression patterns of these proteins in adipocytes.

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LS14: A Novel Human Adipocyte Cell Line that Produces

Given the paucity of suitable human adipocyte cell lines (4- 7), most investigators have been using murine adipogenic cell lines such as 3T3-L1 and 3T3-442A. Although capable of high levels of differentiation, murine adipocytes do not accurately represent the full spectrum of hormonal and metabolic characteristics of human adipocytes .

Human Adipocytes | Biocompare

Your search returned 23 Human Adipocytes Cells and Microorganisms across 2 suppliers. Showing 2 of 2 suppliers (23 products total) > >> Select All. Reliable epithelial cell line for ALI. Read Review. Easy to Use Control for Flow Cytometry.

Cell Models and Their Application for Studying Adipogenic

Recently, new cell lines and protocols have appeared to improve adipocyte culture, such as the OP9 cell line and other models to study the brown/beige adipocytes. However, the 3T3-L1 cells remain to be the most commonly used cell model for studying adipogenesis in vitro because the protocols for these cells are highly developed and standardized.

Co-culture With Human Breast Adipocytes Differentially

Transwell co-culture of human breast adipocytes with breast cancer cell lines. Transwell inserts (Transwell ® permeable supports 0.4 μm polyester membrane 12 mm insert, costar ®, Corning) were soaked in DMEM/F12, 10% FBS media at 37˚C for ≥1 h. Differentiated adipocytes (on the bottom of each well in the 12-well plates) were replenished

Adipose Cells | Creative Bioarray

The classic cell types for studying adipogenesis are established cell lines, such as the 3T3-L1 cell line, which are developed by clonal expansion of rodent-derived cells and only contain a single cell type. Although often thought to be immortal, the ability of 3T3-L1 cells to differentiate into adipocytes decreases with increasing number of

Primary Subcutaneous Pre-adipocytes; Normal, Human | ATCC

ATCC® Normal Human Pre-Adipocytes are derived from de-differentiated mature adipocytes by ceiling culture and provide an ideal culture model for the study of diabetes, obesity, metabolism, insulin sensitivity, and adipose biology. Human Pre-Adipocytes can be expanded in an undifferentiated state for future differentiation to mature Adipocytes and show higher efficiency of adipogenesis

Is there a representative cell line of human visceral

There are 2 known human adipocyte cell lines, Lisa-2 from liposarcoma and the SGBS which we have used from SC fat of WAT from infant. SGBS has a good adipose differentiation even upto 50th passage.

The SGBS cell strain as a model for the in vitro study of

Introduction: The murine adipocyte cell line 3T3-L1 is well characterised and used widely, while the human pre-adipocyte cell strain, Simpson-Golabi-Behmel Syndrome (SGBS), requires validation for use in human studies. Obesity is currently estimated to account for up to 41 % of the worldwide cancer burden. A human in vitro model system is required to elucidate the molecular mechanisms for this

Human Preadipocytes: HPAd, adult subcutaneous – Sigma-Aldrich

General description. Most research in adipocyte biology was conducted in mouse 3T3-L1 cell lines. It has been impossible to use human adipocytes because they die within 24 hours after isolation. Primary Human Preadipocytes (HPAd) are derived from human subcutaneous adipose tissue at various sites and adipose depot on the heart.

Human White Preadipocytes (HWP) – PromoCell

Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request). Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes

Human Cells – ATCC

Human cell lines are a critical biological resource. These in vitro models of human biology are used in biomedical research, toxicology studies, bioindustry, drug discovery, vaccine development, protein production, and a host of other applications. To fulfill this enormous need, ATCC offers the largest collection of cells in the world, with

Preadipocyte Cell Culture – PromoCell

Preadipocyte Cell Culture. Primary human preadipocytes isolated from subcutaneous or visceral fatty tissue of individual donors. Our complete adipocyte media portfolio ensures optimal growth, differentiation and maintenance of these cells. Human preadipocytes, as well as growth and maintenance media – everything you need for your research

The human cell lines – The Human Protein Atlas

The Cell Line section contains information on genome-wide RNA expression profiles of human protein-coding genes in 69 human cell lines. The transcriptomics analysis includes classification based on specificity, distribution and expression cluster analysis across all cell lines. More information about the specific content and the generation and

Studying Adipose Tissue in the Breast Tumor

For researchers looking for a quick means to produce adipocytes where using human cells is not a high priority, the mouse cell line OP9 achieves mature adipocytes in 3 days of differentiation . Culture platform throughput. Throughput is another potential area for innovation in adipocyte-BC culture models.

StemPro™ Human Adipose-Derived Stem Cells

Human Adipose Derived Stem Cells (ADSCs) are isolated from human lipoaspirate tissue and cryopreserved from primary cultures. Before being cryopreserved the ADSCs are expanded for one passage in MesenPRO RS™ Medium (Cat. no. 12746-012) – a revolutionary new reduced serum (2%) medium that results in reduced ADSC doubling times.

Direct conversion of human fibroblasts to brown adipocytes

After reaching 80-90% confluence of each human fibroblast line, the medium was changed to start direct conversion into brown adipocyte-like cells with adipocyte medium prepared from high-glucose

Human Pre-Adipocyte Cells | Creative Bioarray

Human Pre-Adipocytes can be expanded in an undifferentiated state for future differentiation to mature Adipocytes. Creative Bioarray’s Human Pre-Adipocytes may also be differentiated down chondrogenic, and osteogenic lineages. Cell Features: HMSC-Ad are isolated from adult lipoaspirate and are cryopreserved as secondary cells.

PAZ6 Cells Constitute a Representative Model for Human

A human brown adipocyte cell line is therefore critical in broadening the options available to researchers in the field. The human BAT-cell line PAZ6 was created to address such a need and has been well characterized by several research groups around the world.

SGBS cells as a model of human adipocyte browning: A

The Simpson Golabi Behmel Syndrome (SGBS) pre-adipocyte cell strain is widely considered to be a representative in vitro model of human white pre-adipocytes. A recent study suggested that SGBS

Human Adipocyte Cell Culture – T150 Plated Cells – Celprogen

Human Adipocyte Primary Cell Culture – T150 Plated Cells. Also Avaliable as: Frozen Vial Cells shipped with Dry-ice. Cat#36065-01 T25 plated Cells shipped at room temperature. Cat#36065-01-T25 T75 plated Cells shipped at room temperature. Cat#36065-01-T75. 120 Population doublings or up to 12 passages.

Human All Cell Lines Bv173 | ATCC | Bioz

Article Snippet: Cell culture All human ALL cell lines (BV173, RS4;11, and Nalm6) and murine pre-adipocyte 3T3-L1 were from ATCC. Techniques: Confocal Microscopy, Fluorescence, Transformation Assay, Expressing, Real-time Polymerase Chain Reaction, Western Blot

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Established human brown adipocyte line and method for

US-2011117066-A1 chemical patent summary.

PDF Mature Human White Adipocytes Cultured under – Cell

research on adipocytes is conducted almost exclusively using adipocytes differentiated in vitro from precursors. Primary prea-dipocytes and, more commonly, the mouse cell lines 3T3-L1, 3T3-F442A, and C3H10T1/2, which have been immortalized from non-adipose precursors but nevertheless possess adipo-

Characterisation of adipocyte-derived extracellular

The murine adipocyte cell line 3T3-L1 has also been reported to produce EVs containing adipokines (2, 3) and factors that promote angiogenesis in vivo . Human adipose tissue explants have also been shown to produce EVs that can modulate monocyte differentiation and alter insulin signalling in adipocytes ( 14 ) and liver cells ( 15 ).

Adipocyte – Wikipedia

Adipocytes, also known as lipocytes and fat cells, are the cells that primarily compose adipose tissue, specialized in storing energy as fat. Adipocytes are derived from mesenchymal stem cells which give rise to adipocytes through adipogenesis.In cell culture, adipocyte progenitors can also form osteoblasts, myocytes and other cell types.. There are two types of adipose tissue, white adipose

Comprehensive molecular characterization of human

Cell lines/cell culturing. The brown pre-adipocyte cell line PAZ6 was kindly gifted by Dr. D. Strosberg [] and SGBS cells were kindly provided by Dr. M. Wabitsch [].SGBS pre-adipocytes were grown in DMEM/F12 medium supplemented with 10% fetal calf serum (FCS), 1.7 mM panthotenat, 3.3 mM biotin and 1% penicillin/streptomycin (pen-strep).

Cellosaurus cell line LiSa-2 (CVCL_M821)

Derived from metastatic site: Retroperitoneal space. LiSa-2, a novel human liposarcoma cell line with a high capacity for terminal adipose differentiation. Comparative expression analysis of isolated human adipocytes and the human adipose cell lines LiSa-2 and PAZ6. Characterization of liposarcoma cell lines for preclinical and biological studies.

US9790469B2 – Established human brown adipocyte line and

The subject matter of the invention is a functional population of human brown adipocytes, in which the expression of UCP1, CIDEA, CPT1B and Bc12 is higher, the expression of Bax is lower and the expression of PPAR-alpha, PGC-1alpha, PGC-1 beta and PRDM16 is similar compared with the corresponding expressions of a population of human white adipocytes.

US20110117066A1 – Established human brown adipocyte line

The subject matter of the invention is a functional population of human brown adipocytes, in which the expression of UCP1, CIDEA, CPT1B and Bcl2 is higher, the expression of Bax is lower and the expression of PPAR-alpha, PGC-1alpha, PGC-1beta and PRDM16 is similar compared with the corresponding expressions of a population of human white adipocytes.

Spatial mapping reveals human adipocyte subpopulations

Tissue microarchitecture and cell composition are major determinants of organ function. Here, Bäckdahl et al. apply spatial transcriptomics to human white adipose tissue. This reveals that the tissue is more spatially defined than expected and identifies three distinct mature adipocyte subtypes with qualitatively different sensitivities to insulin stimulation in vivo.

Functional Human Beige Adipocytes From Induced Pluripotent

Beige adipocytes are likely present, albeit in small amounts, in various human adipose depots and may constitute an important subset of thermogenic cells in adults (2,3). The scalability of our protocol makes it a valuable tool to provide an unlimited source of cells to model beige adipose tissue development and physiology, and for metabolic

Omental adipocytes promote peritoneal metastasis of

Human microvascular endothelial cells (HMVECs) (Lonza, Basel, Switzerland) and human omental precursor adipocytes (OmPrAd) (Zen-Bio, Inc., Research Triangle, NC) were also used in this study.

Human immortalized brown adipocytes express functional

Human brown pre-adipocytes were immortalized by microinjection of the genes encoding simian virus 40 T and t antigens under the control of the human vimentin promotor. The transfected pre-adipocytes were cultured for several months with no loss of their morphological characteristics. These cells accumulate lipids and differentiate into adipocytes when treated with insulin, triiodothyronine and

Adipocyte Differentiation and Gene Expression | The

The 3T3-L1 cell line is one of the most well-characterized and reliable models for studying the conversion of preadipocytes into adipocytes. When injected into mice, 3T3-L1 preadipocytes differentiate and form fat pads that are indistinguishable from normal adipose tissue ( Green and Kehinde 1979 ).

Adipocyte-derived exosomes may promote breast cancer

To investigate the role of adipocytes in breast cancer progression, we first used a transwell system to coculture the human ER-positive cell line Michigan Cancer Foundation-7 (MCF7) with human adipocytes that had been differentiated from primary preadipocytes surgically obtained from cancer-free, female patients with or without T2D undergoing

D-Chiro-Inositol Regulates Insulin Signaling in Human

Since it is unclear whether D-Chiro-Ins directly acts on adipocytes, we aimed to study D-Chiro-Ins’s actions on adipocyte viability, proliferation, differentiation, and insulin-related protein expression using a human adipocyte cell line derived from Simpson-Golabi-Behmel Syndrome (SGBS) which fully differentiates to mature adipocytes.

(PDF) Comprehensive molecular characterization of human

With regards to brown pre- or adipocyte cell lines, there are no other immortalized human cell lines to our knowledge. However, human multipotent adipose-derived stem cells (MADS) that have the potential to differentiate in either white or brown fat adipocytes have been established in culture [32].

PDF Clonal derivation of white and brown adipocyte progenitor

RESEARCH Open Access Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells Michael D. West1, Ching-Fang Chang3, Dana Larocca1*, Jie Li1, Jianjie Jiang1, Pamela Sim1, Ivan Labat1, Karen B. Chapman2, Kari E. Wong4, James Nicoll5, Michael J. Van Kanegan5, Aubrey D. N. J. de Grey1,6, Igor O. Nasonkin7, Andreas Stahl3 and Hal Sternberg1

Characterization of adipocyte differentiation from human

Adipocyte hyperplasia is associated with obesity and arises due to adipogenic differentiation of resident multipotent stem cells in the vascular stroma of adipose tissue and remote stem cells of other organs. The mechanistic characterization of adipocyte differentiation has been researched in murine pre-adipocyte models (i.e. 3T3-L1 and 3T3-F442A), revealing that growth-arrest pre-adipocytes

PDF Comprehensive molecular characterization of human

Cell lines/cell culturing The brown pre-adipocyte cell line PAZ6 was kindly gifted by Dr. D. Strosberg [20] and SGBS cells were kindly provided by Dr. M. Wabitsch [19]. SGBS pre-adipocytes were grown in DMEM/F12 medium supple-mented with 10% fetal calf serum (FCS), 1.7 mM panthotenat, 3.3 mM biotin and 1% penicillin/strepto-mycin (pen-strep).

CD36 Is a Marker of Human Adipocyte Progenitors with

currently no immortalized adipocyte cell lines available (with the exception of a cell line derived from an infant with the Simpson-Golabi-Behmel syndrome, SGBS [17]). Identifying cel-lular markers of human APs with pronounced adipogenic/TG storage ability could enable the establishment of valuable cel-

Rapamycin Inhibits Human Adipocyte Differentiation in

ways activated by insulin that induce human adipocyte differentiation are incompletely understood. The 3T3-L1 murine preadipocyte cell line is a reliable model of adipocyte differentiation, a process that requires insulin or insulin growth factor-1 (3,5). Although it was suggested that insulin acts through preadipocyte insulin

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Characterization of immortalized human brown and white pre

The molecular circuitry of mouse brown adipocyte differentiation and function has been studied intensely due to easily accessible primary cell cultures and the existence of a number of useful cell models. The selection of human adipocyte cell models derived from adult BAT is limited, thereby hampering studies of the molecular control of human

Hypertrophied human adipocyte spheroids as in vitro model

Adipocyte enlargement is a key feature of obesity and associated with insulin resistance and metabolic disease; The cause and consequences of adipocyte enlargement have remained hard to study in vitro due to a lack of human cell models with representative morphology; This paper provides an easily set up spheroid culture method, HUVAS (human unilocular vascularized adipocyte spheroids), for the

In Depth Quantitative Proteomic and Transcriptomic

Preadipocyte cell lines, such as murine 3T3-L1, or primary adipocytes from mice, rats or humans serve as experimental models for human adipogenesis and are widely used to characterize adipogenic regulators and thereby provide substantial insights into the molecular processes regulating adipogenesis.

Human Preadipocytes Inhibit Proliferation of Mcf-7 Breast

In this study, we compared the proliferation of MCF-7 breast-cancer cell line in culture with or without presence of primary isolated human adipocytes and preadipocytes by MTT colorimetric assay in 2, 4 and 6 days of culture experiments. Human adipocytes increased the proliferation of MCF-7 cells in the co-culture about 8.6%, 12% and 12% more

PDF Supplementary Appendix Aug19 acceptall – NEJM

Experimental validation of enhancer activity in a human adipocyte cell line. _____ 5 c. Validation of genetic control and narrowing down candidate region using enhancer assays in risk and non-risk

LiSaâ 2, a novel human liposarcoma cell line with a high

report a permanent human PPAR-g-expressing liposarcoma cell line, LiSa-2, derived from a poorly differentiated, pleomorphic liposarcoma. Grown in adipogenic medium, LiSa-2 cells differen-tiate into adipocyte-like cells with several phenotypic, biochemical and functional characteristics of mature human fat cells. MATERIAL AND METHODS Cell culture

Primary Culture of Human Adipocyte Precursor Cells

Although clonal cell lines are valuable tools for the identification of mechanisms associated with proliferation or differentiation such models do not necessarily represent the complexity of adipose tissue physiology. Pfeiffer E.F. (1989) Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells in a

Immortalized cell lines from human adipose tissue, process

The cell lines are formed of pre-adipocytes containing a nucleic acid fragment including at least one fragment immortalizing a viral oncogene, and at least one promoter selected from the group containing a promoter of said viral oncogene and a human vimentine gene regulatory region fragment.

EHD2 regulates adipocyte function and is enriched at cell

Perilipin-1, one of the first identified lipid droplet-associated protein (Greenberg et al., 1991), was recently proposed to segregate into protein microdomains at the cell surface of human adipocytes (Hansen et al., 2017). Still, no further description of the mechanistic function, or relation between these protein domains and caveolae or

3D Adipocyte Culture – CherryBiotech Innovation – Cubix

Under the microscope, the adipocyte appears bloated with triglycerides. The nucleus of the cell is displaced to one side by the fat. The cytoplasm of the cell looks like a thin line surrounding the pool of fat. Can human adipose tissue be reproduced in a laboratory? It can now.

Megakaryocytes and platelets from a novel human adipose

Thus, we developed a manufacturing system for platelets from a donor-independent cell source: a human adipose-derived mesenchymal stromal/stem cell line (ASCL). The ASCL was obtained using an upside-down culture flask method and satisfied the minimal criteria for defining mesenchymal stem cells (MSCs) by The International Society for Cellular

Insulin/glucose induces natriuretic peptide clearance

human adipocyte cell line obtained from Simpson-Golabi-Behmel syndrome (SGBS) (13). SGBS is a well-reproducible in vitro model of human adipocytes to test insulin and the NP system as well as for glucose-regulated gene analysis, whereas human VAT-derived primary preadipocytes are the more closely related model to in vivo visceral adipocytes

Clonal derivation of white and brown adipocyte progenitor

The transcriptome of the human hES-derived clonal embryonic progenitor cell lines E3, C4ELS5.1, NP88, and NP110 representing three class of definitive adipocyte progenitors were compared to the relatively non-adipogenic line E85 and adult-derived BAT and SAT-derived cells using gene expression microarrays, RT-qPCR, metabolic analysis and

T37i Mouse Brown Adipocyte Cell Line | SCC250

The T37i cell line is a rapidly proliferating brown preadipocyte cell line derived from a mouse hibernoma. Differentiation of T37i cells into brown adipocytes occurs when incubated with triiodothyronine (T3) and insulin over 3-6 days. Differentiation can also be induced by incubation with steroid hormones such as aldosterone, which induces

Characterisation of aromatase expression in the human

Aromatase expression in SGBS cells. (a) RT-PCR of total RNA from SGBS cells treated with vehicle (ethanol; 0.001%) or forskolin (FSK; 25 μM) and PMA (4 nM).Human primary adipocyte cells (hPA). (b) Quantification of total aromatase mRNA levels in SGBS cells treated with vehicle (ethanol; 0.001%) or forskolin (FSK; 25 μM) and PMA (4 nM) for 24h. cDNA was amplified by real-time PCR against

A2780 Cell Line human 93112519, ovarian carcinoma | Sigma

Cell Line Description. The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (Sigma catalogue no. 93112517) and the adriamycin resistant cell line A2780 ADR

Retinoic acid has different effects on UCP1 expression in

To investigate if chronic treatment with ATRA had the same effects on human adipocytes as observed with mouse adipocytes, we exposed the human white preadipocyte cell line SGBS , human multipotent adipose-derived stem cells (hMADS) [45, 46] and primary human white preadipocytes to various concentrations of ATRA during differentiation (Figure 7A

Inflammatory Role of Toll-Like Receptors in Human and

The expression level of TLRs in 3T3-L1 cell line has not to be compared to what was obtained from human samples since adipose tissue is constituted of numerous different cell types (e.g., adipocytes, macrophages, and endothelial cells).

Differentiation of 3T3-L1 cells into adipocyte-like cells

The mouse embryonic fibroblast cell line 3T3-L1 can be chemically induced to differentiate into adipocyte-like cells. 3T3-L1 differentiation is an economical and convenient way to generate adipocyte-like cells for experiments. In this case study, we tested adipocyte characteristics such as lipid accumulation, production of leptin and adipocyte

Characterization of lipid metabolism in insulin-sensitive

Abstract. There is a great demand for cell models to study human adipocyte function. Here we describe the adipogenic differentiation of a telomerase-immortalized human mesenchymal stem cell line (hMSC-Tert) that maintains numerous features of terminally differentiated adipocytes even after prolonged withdrawal of the peroxisome proliferator activated receptor {gamma} (PPAR{gamma}) agonist

Autocrine Action of Adiponectin on Human Fat Cells

Effect of adiponectin on insulin signaling in human skeletal m