What is aptamer used for?
What is aptamer used for?
Like monoclonal antibodies, aptamers can be used for the molecular recognition of their respective targets. Aptamers have been successfully used for pathogen recognition, cancer recognition, monitoring environmental contamination, and as stem cell markers.Mar 7, 2019
What is aptamer sensor?
Aptamers are short DNA or RNA oligonucleotides with the capability to bind selectively specific target molecules.
Are aptamers synthetic antibodies?
What is an aptamer? Aptamers (synthetic antibodies) are (stable) single-stranded DNA, RNA, or peptide molecules capable of binding to its target antigen with high affinity and specificity.
What is the aptamer sequence?
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization.Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular bindingmolecular bindingLigand efficiency is a measurement of the binding energy per atom of a ligand to its binding partner, such as a receptor or enzyme.https://en.wikipedia.org › wiki › Ligand_efficiencyLigand efficiency – Wikipedia. They are isolated via SELEX (Systematic Evolution of Ligands by Exponential EnrichmentSystematic Evolution of Ligands by Exponential EnrichmentSystematic Evolution of Ligands by Exponential Enrichment (SELEX) is an in vitro process enabling selection of nucleic acid molecules binding to target ligands with high binding affinity and specificity.https://pubmed.ncbi.nlm.nih.gov › Cell-SELEX: in vitro selection of synthetic small specific ligands), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization.24-Nov-2017
Can aptamers bind antibodies?
Aptamers, which can bind universally on Fc regionFc regionThe fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system.https://en.wikipedia.org › wiki › Fragment_crystallizable_regionFragment crystallizable region – Wikipedia of antibodies can be selected with target replacement strategy coupled to conventional SELEX method, i.e., to achieve universal binding capability, multiple immunoglobulin subclasses have to be consecutively substituted in a single SELEX process [5].
What is a DNA aptamer?
Aptamers are a special class of nucleic acid molecules that are beginning to be investigated for clinical use. These small RNA/DNA molecules can form secondary and tertiary structures capable of specifically binding proteins or other cellular targets; they are essentially a chemical equivalent of antibodies.
What is the difference between aptamer and antibody?
They are in general more stable than antibodies, and have a longer shelf life. Aptamers are produced through a simple and inexpensive process and the time required to generate aptamers is comparatively short. Unlike antibodies, aptamers do not need animals or an immune response for their production.
Are aptamers antibodies?
Aptamers are produced through a simple and inexpensive process and the time required to generate aptamers is comparatively short. Unlike antibodies, aptamers do not need animals or an immune response for their production.
What is a RNA aptamer?
RNA Aptamers are defined as RNA oligonucleotides that bind to a specific target with high affinity and specificity, similarly to how an antibody binds to an antigen.
What is aptamer molecule?
Aptamers are short, single-stranded DNA or RNA (ssDNA or ssRNA) molecules that can selectively bind to a specific target, including proteins, peptides, carbohydrates, small molecules, toxins, and even live cells. Aptamers assume a variety of shapes due to their tendency to form helices and single-stranded loops.
What can aptamers do?
Aptamers are single-stranded oligonucleotides that fold into defined architectures and bind to targets such as proteins. In binding proteins they often inhibit protein–protein interactions and thereby may elicit therapeutic effects such as antagonism.
What is the SELEX process?
Background. SELEX is an iterative process in which highly diverse synthetic nucleic acid libraries are selected over many rounds to finally identify aptamers with desired properties. However, little is understood as how binders are enriched during the selection course.
What is an aptamer how is it selected and how is it used as a therapeutic agent?
They are selected against target molecules by an iterative process known as SELEX (Systematic Evolution of Ligands by Exponential Enrichment) [1], which was developed in 1990. Due to its various advantages, aptamers are regarded as promising alternatives to antibodies.Jun 1, 2016
Are aptamers better than antibodies?
Aptamers offer significant advantages over antibodies [8]. They are in general more stable than antibodies, and have a longer shelf life. Aptamers are produced through a simple and inexpensive process and the time required to generate aptamers is comparatively short.
What is an Aptasensor?
Aptasensors are biosensors that use aptamers as recognition element. Aptamers are single-stranded DNA or RNA molecules that bind target molecules with high affinity and specificity.
Can aptamers bind to DNA?
Aptamers are single-stranded, synthetic oligonucleotides (DNA or RNA) which fold into 3-dimensional shapes capable of binding non-covalently and with high affinity to a target molecule.
Can aptamers replace antibodies?
While some minor amendments to the existing IHC protocols may be necessary depending on the aptamer or the target biomarker, ultimately, aptamers can replace traditional antibodies in such applications with very limited capital expenditure, additional resources/reagents, or staff training being required.
What is the difference between aptamer and oligonucleotide?
Aptamers are the oligonucleotide chains with higher specificity and affinity towards target protein. Thus, aptamers are considered as a nucleic acid version of antibodies. Various antibodies mediated methods has adopted aptamer-based detection technology.
How big is an aptamer?
20 to 60 nucleotides
What are biosensors used for?
Biosensors are used for the detection of pathogens in food. Presence of Escherichia coli in vegetables, is a bioindicator of faecal contamination in food. E. coli has been measured by detecting variation in pH caused by ammonia (produced by urease–E.Jan 6, 2016
Aptamers: Problems, Solutions and Prospects – PMC
Currently, a large number of generated aptamers can bind various targets, ranging from simple inorganic molecules to large protein complexes, and entire cells. In fact, aptamers are nucleotide analogues of antibodies, but aptamer-generation is significantly easier and cheaper than the production of antibodies [ 6, 7 ].
Coupling Aptamer-based Protein Tagging with Metabolic
Exosomal glycoproteins play important roles in many physiological and pathological functions. Herein, we developed a dual labeling strategy based on a protein-specific aptamer tagging and metabolic glycan labeling for visualizing glycosylation of specific proteins on exosomes. The glycosylation of e …
PPAI: a web server for predicting protein-aptamer
thanks to the rapid accumulation of protein-aptamer interaction data, it is necessary and feasible to construct an accurate and effective computational model to predict aptamers binding to certain interested proteins and protein-aptamer interactions, which is beneficial for understanding mechanisms of protein-aptamer interactions and improving …
Overview of the Therapeutic Potential of Aptamers
Aptamers are single-stranded nucleic acids, either DNA or RNA, that bind to their target protein with high affinity and specificity. During the early years of aptamers technology, RNA aptamers were preferred since RNA was assumed to fold in a more functional domain. However, recently, DNA aptamers are becoming more prevalent [ 1 ].
Aptamers targeting cell surface proteins
Aptamers targeting cell surface proteins High affinity binders targeting specific cell surface proteins are vital for development of basic and applied biosciences. However, despite sustained efforts to generate such binders by chemicals and antibodies, there are still many cell surface proteins that lack high affinity binders. Nucleic acid …
Protein-based SELEX Services for Aptamer Development
Aptamers are short synthetic single-stranded (ss) DNA or RNA sequences that can specifically bind to a variety of substances such as proteins, chemical compounds, cells, and micro-organisms. They have been used in various fields of biology and medicine such as targeted gene therapy and drug delivery.
Aptamer – Wikipedia
Aptamers (from the Latin aptus – fit, and Greek meros – part) are oligonucleotide or peptide molecules that bind to a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool, but natural aptamers also exist in riboswitches.
PPAI: a web server for predicting protein-aptamer
The aptamers have the merits of easy synthesis and good stability, their specific bindings to proteins play an important role in various life activities of the organisms. Although the experimental aptamer screening technology has been further developed recently, it still has more disadvantages such as time-consuming, expensive and labor-intensive.
Aptamers, the bivalent agents as probes and therapies for
Aptamer, one of the novel DNA receptors, is a single-stranded folded RNA or ssDNA that can bind and detect various nucleic and non-nucleic acid molecules with high affinity and specificity. The application of aptamer probes for virus detection has increased in recent years.
Identification and Characterization of DNA Aptamers
When measuring the binding of aptamers with his-tag Tau441 protein (SignalChem), the experimental layout was the same as that described above, except that the loading concentration of his-tag Tau441 protein was 1 μg/mL supplemented with 0.75 mM imidazole, and the concentrations for aptamer dilutions were 0.75 μM, 1.5 μM, 3 μM, 6 μM, and 12
Protein Labeling and Crosslinking by Covalent Aptamers
Protein Labeling and Crosslinking by Covalent Aptamers Protein Labeling and Crosslinking by Covalent Aptamers Angew Chem Int Ed Engl. 2021 Apr 29. doi: 10.1002/anie.202101174. Online ahead of print. Authors Yaniv Tivon 1 , Gianna Falcone 1 , Alexander Deiters 2 Affiliations 1 University of Pittsburgh, Chemistry, UNITED STATES.
A universal protein tag for delivery of SiRNA-aptamer chimeras
The protein selectively tags along the siRNA block of individual chimera, rendering the overall size of the complex small, desirable for deep tissue penetration, and the aptamer block accessible for target recognition.
AptaNet as a deep learning approach for aptamer-protein
Aptamers are short oligonucleotides (DNA/RNA) or peptide molecules that can selectively bind to their specific targets with high specificity and affinity. As a powerful new class of amino acid
Peptide aptamers: specific inhibitors of protein function
Peptide aptamers can be used in order to validate therapeutic targets at the intracellular level. Moreover, the peptide aptamer molecules themselves should possess therapeutic potential, both as lead structures for drug design and as a basis for the development of protein drugs. Publication types Research Support, Non-U.S. Gov’t Review MeSH terms
Using modified aptamers for site specific protein-aptamer
In both physiological environments and buffer solutions, protein-aptamer interactions are bioorthogonal, and aptamers can recognize and bind to their target proteins with high affinity, providing PAT with the requisite orthogonality ( Fig. 1b ).
Peptide Aptamers: Development and Applications – PMC
Peptide aptamers are small combinatorial proteins that are selected to bind to specific sites on their target molecules. Peptide aptamers consist of short, 5-20 amino acid residues long sequences, typically embedded as a loop within a stable protein scaffold.
Anti-SARS-CoV-2 N-Protein Aptamer – RayBiotech
Aptamer selection was done against recombinant SARS-CoV-2 protein. Before use, incubate the aptamer at 95°C for 15 minutes before removing from heat and allowing to cool to room temperature. Avoid multiple foldings by calculating the required amount of aptamer and folding the volume needed in a separate tube. Protein Information
Aptamer-based optical manipulation of protein subcellular
An aptamer (Apt) that could specifically recognize RelA protein with high binding affinity (Kd = 0.64 nM) 26 is used as the recognition unit. To impart spatiotemporal regulation on target protein,
Identification of SARS-CoV-2-against aptamer with high
a The binding between aptamers of nCoV-S1-Apt1 ~6 (0.2 μM) and S1 protein (2 μM) was characterized by CZE. “☀” and ” ” indicate the two forms complex peaks formed by aptamer and S1
Aptamer-based protein inhibitors | Request PDF
Request PDF | Aptamer-based protein inhibitors | Nucleic acid aptamers have been discovered in nature and in the laboratory that can bind and block that activity of numerous target proteins. They
Aptamer binding assays for proteins: The thrombin example
Through aptamer-protein interactions, femtomole amounts of thrombin were also detected by a four-chamber microfluidic biochip with the technique of SPRI. Jung et al. [108] constructed a surface acoustic wave (SAW) sensor chip by forming thrombin-sensitive biofunctionalized surfaces through immobilization of an RNA aptamer for thrombin.
Apta-Index™ (Aptamer Database) – Library of 500+ Aptamers
Apta-Index™. Aptagen is the world’s leader in aptamers. We offer the Apta-index, the most advanced user-friendly database on aptamers. Aptagen does not list this information contained herein as products but as a database of information obtained from the published literature. If your lab has developed aptamer (s), we would like to hear from you.
Analysis of aptamer discovery and technology | Nature
The structure of an α-thrombin-aptamer complex (shown in part c), which includes the protein and the complementary aptamer, was solved by solution NMR (Protein Data Bank identifier: 3DD2) 129
Prediction of aptamer-protein interacting pairs based on
Aptamer-protein interacting pairs play important roles in physiological functions and structural characterization. Identifying aptamer-protein interacting pairs is challenging and limited, despite the tremendous applications of aptamers. • A sparse autoencoder was used to characterize features for the target protein sequences. •
Protein Labeling and Crosslinking by Covalent Aptamers
These aptamers are generated through introduction of a proximity-driven electrophile at specific nucleotide sites. Using thrombin as a proof-of-concept, we demonstrate that covalent aptamers can selectively transfer a variety of functional handles and/or irreversibly crosslink to the target protein.
Synthetic Translational Regulation by Protein-Binding RNA
Rational design approaches for the regulation of gene expression are expanding the synthetic biology toolbox. However, only a few tools for regulating gene expression at the translational level have been developed. Here, we devise an approach for translational regulation using the MS2 and PP7 aptamer and coat-protein pairs in Escherichia coli. The aptamers are used as operators in
AptamerProteinPrediction – 39.96.85.9
AptamerProteinPrediction. The interactions between proteins and aptamers are prevalent in organisms and play an important role in various life activities. Thanks to the rapid accumulation of protein-aptamer interaction data, it is very important and feasible to construct an accurate computational model to predict protein-aptamer interactions
Designing of peptide aptamer targeting the receptor
Plasma protein is another constituent of blood plasma, and serum albumin is a major plasma protein. The peptide aptamer binds with the HSA with a binding energy of − 6.6 kcal/mol, which is much higher than the binding energy of aptamer-RBD of S-protein complex (− 11.3 kcal/mol).
What are Peptide Aptamers? – News-Medical.net
Protein aptamers have therefore been used in many research fields, for example for the identification of genes, which are involved in the cell cycle. Further developments have also shown that these
Aptamers – AMSBIO
As well as biotinylated aptamer, we supply kits with FITC dye conjugated biotin aptamer, allowing you to perform the cell isolation and flow cytometry analysis simultaneously. Our bi-labeled aptamers have FITC at the 5′-end to monitor protein expression by flow cytometry and biotin at the 3′-end to separate target cells.
Aptamer-Based Affinity Chromatography for Protein
Aptamer-Protein Interaction. Literature related to aptamer-protein interactions in affinity chromatography is almost nonexistent. A RNA or DNA sequence is not a simple linear primary structure, but, as mentioned above, a macromolecule subject to secondary and tertiary configurations [69, 77] (see also Sect. 3.2).
Signaling Aptamer/Protein Binding by a Molecular Light
A novel method of signaling aptamer/protein binding for aptamer-based protein detection has been developed using a molecular light switch complex, [Ru(phen)2(dppz)]2+. The method takes advantage of the sensitive luminescence signal change of [Ru(phen)2(dppz)]2+ intercalating to the aptamer upon protein/aptamer binding. A 37-nt DNA aptamer against immunoglobulin E (IgE) was first tested as a
Aptamer/AuNP Biosensor for Colorimetric Profiling of
The sensor was designed by the noncovalent conjugation of 13-nm AuNPs with a panel of five aptamers previously demonstrated to target cell-surface proteins with high specificity and affinity (Figure 1). []The aptamers can bind exosome surface proteins and induce the aggregation of AuNPs for exosomal protein profiling.
Aptamer-Based Proteomic Platform Identifies Novel Protein
Instances of cross-reactivity, nonspecific binding, and sequence variation in the aptamer binding sites have been described with the SomaScan platform, which may limit specificity and precision of protein quantification. 43 SomaScan aptamers for 4 of the 6 proteins associated with incident HF in our study (TSP2, MBL, ErbB1, and NT-proBNP) have
Aptamers to SARS-CoV-2 Spike and N Protein – Creative Biolabs
The aptamer is composed of DNA or RNA (mainly DNA), which is smaller than the protein. After screening and enrichment by SELEX, it can have sensitivity comparable to antigen-antibody reaction, and it is easier to synthesize and has better stability.
Aptamers targeting protein-specific glycosylation in tumor
Here we report a method for selecting aptamers toward the glycosylation site of a protein. It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern.
Aptamer for the Histidine Tag and an Improved Method for
The Berkeley Lab method generates aptamers against the protein portion of a His-tagged protein. These aptamers can then be used for the detection, purification, and capture of native proteins. The improved method is less labor intensive, less expensive, easily scalable, and increases the utility of the aptamers in downstream applications.
Aptamer-Based Western Blot for Selective Protein Recognition
Selective protein recognition is critical in molecular biology techniques such as Western blotting and ELISA. Successful detection of the target proteins in these methods relies on the specific interaction of the antibodies, which often bring a high production cost and require a long incubation time. Aptamers represent an alternative class of simple and affordable affinity reagents for protein
Bispecific Aptamer Induced Artificial Protein-Pairing: A
Herein, we develop a strategy, called bispecific aptamer induced artificial protein-pairing, to selectively regulate receptor function. In this strategy, bispecific aptamer probes act as molecular mediators to bind to both a target receptor protein and a paired protein, which brings the two proteins into close proximity on the living cell membrane.
Synergistic insights into human health from aptamer- and
Relative protein abundances were measured in fasting EDTA-plasma samples from 12,084 Fenland Study participants collected at the baseline visit by SomaLogic Inc. (Boulder, US) using an aptamer
DNA aptamer against EV-A71 VP1 protein: selection and
Three DNA aptamers with high specificity and affinity for EV-A71structual protein VP1 were screened out. A rapid chemiluminutesescence aptamer biosensor for EV-A71 detection was designed out. The selected aptamers could inhibit the RNA replication and protein expression of EV-A71 in RD cells and ameliorate the cytopathogenic effects.
Coupling Aptamer‐based Protein Tagging with Metabolic
A dual labeling strategy for in situ visualization of exosomal protein-specific glycosylation based on a protein-specific aptamer tagging and metabolic glycan labeling (ExoAp-MGL) was used to study the presence and function of exosomal protein-specific glycosylation.
Predicting aptamer sequences that interact with target
To feed aptamer and protein sequences into our classification model, the sequences were encoded into a numerical representation. While most machine learning models generally use a feature vector of a fixed size as an input, aptamer and protein sequences are of variable lengths.
An aptamer for recognizing the transmembrane protein PDL-1
Programmed death-ligand 1 (PD-L1) is overexpressed in a variety of cancer cells. Using protein SELEX, aptamers against PD-L1 were identified. After 10 rounds of selection, 2 aptamers (Apt5 and Apt33) were chosen. Due to the higher specificity and affinity of Apt5 for PD-L1, this aptamer was characterized in detail. The ATTO 647 N-labeled aptamer readily internalizes into PD-L1 positive A2780
Identification and characterization of DNA aptamers
Aptamers have emerged as potential alternative to antibodies as affinity reagents in diagnostic field. Present study was aimed to select and validate ssDNA aptamers specific to CPV. Systematic evolution of ligands through exponential enrichment (SELEX) method was employed for selection of CPV structural protein (VP2) specific DNA aptamers.
Anti-thrombin aptamers – Wikipedia
The aptamer TBA (also known as G15D, HTQ, HD1 or ARC183) is a 15-mer single-stranded DNA with the sequence 5 ‘-GGTTGGTGTGGTTGG-3 ‘. It interacts with the exosite I of human alpha-thrombin, which is the binding site of fibrinogen, so this aptamer acts as an anti-coagulant agent inhibiting the activation of fibrinogen as well as platelet aggregation.In addition, TBA shows good affinity and
Advances in aptamer-based sensing assays for C-reactive
C-reactive protein (CRP), a non-specific acute-phase indicator of inflammation, has been widely recognized for its value in clinical diagnostic applications. With the advancement of testing technologies, there have been many reports on fast, simple, and reliable methods for CRP testing. Among these, the aptamer-based biosensors are the focus and hotspot of research for achieving high
In Vitro Selection of a DNA Aptamer Targeting Degraded
Herein, we present a method to overcome this issue, by selecting aptamers against a degraded form of the toxin B protein, which is a marker for diagnosing toxigenic Clostridium difficile infections. This approach has led to isolation of a DNA aptamer that recognizes degraded toxin B, fresh toxin B, and toxin B spiked into human stool samples.
Raptamer Discovery Group – Next Generation Aptamers
Traditional aptamers use just four nucleotides: A, C, G, and T (in the case of DNA aptamers) and A, C, G, and U (in the case of RNA aptamers). Raptamers™ go further. We generate next-generation aptamers with a backbone made up of standard DNA nucleotides, plus a number of amino acid side chains, through a series of custom modifications that
PDF PPAI: a web server for predicting protein-aptamer interactions
diction of protein-aptamer interactions, some computational models have been devel-oped, for example, Li et al. [7] developed a random forest-based protein-aptamer interaction prediction model, Zhang et al. [8] presented a novel model based on the en-semble method in 2016. However, there are still certain limitations in above models.
PDF Protein Detection with Aptamer Biosensors – MDPI
3. Protein biosensor detection principles based on aptamers Biosensors for protein detection mainly involve antibodies, but lately, also aptamers as biological recognition elements in the case of specific detection and enzymes in the case of total protein detection. Aptamers can rival antibodies in a number of applications.
Search for RNA aptamers against non-structural protein of
Protein-aptamer docking studies. The NSP10 was known to be essential for proper functioning of NSP16 and NSP 14 as it stabilizes the methyl donor binding sites [2, 37].In SARS-CoV, the production of NSP10 was found to be 3-6 folds higher than that of NSP14 and NSP16 for simultaneous formation of NSP10-NSP14 and NSP10-NSP16 complexes.
(PDF) Methods for selection of aptamers to protein targets
Basic methods of site-directed aptamer selection. a) Method of competitive elution of aptamers using another ligand binding in the same site of the protein.
Aptamers from Gene Link
Aptamers can distinguish between closely related but non-identical members of a protein family, or between different functional or conformational states of the same protein. In a striking example of specificity, an aptamer to the small molecule theophylline (1,3-dimethylxanthine) binds with 10,000-fold lower affinity to caffeine (1,3,7
What are Peptide Aptamers? – News-Medical.net
Therefore, the protein aptamers that lead to transcription of the reporter gene are the proteins that are complementary to the desired sequence. Alternatively, in the cell-free phage display
Synthetic DNA Aptamers to Detect Protein Molecular
Real-time protein detection in homogeneous solutions is necessary in many biotechnology and biomedical studies. The recent development of molecular aptamers, combined with fluorescence techniques, may provide an easy and efficient approach to protein elucidation.
Detection of SARS-CoV-2 RNA Using a DNA Aptamer Mimic of
These RNAs include the Spinach and Spinach2 aptamers, which bind and activate the fluorescence of fluorophores similar to that found in green fluorescent protein. Although addnl. highly fluorescent RNA-fluorophore complexes would extend the utility of this technol., the identification of novel RNA-fluorophore complexes is difficult.
A serum-stable RNA aptamer specific for SARS-CoV-2
Here we report the selection and characterization of a serum-stable RNA aptamer, RBD-PB6, that binds with nanomolar affinity to the RBD of SARS-CoV-2 spike protein and neutralizes viral infectivity. The aptamer contains 2′-fluoro pyrimidine modifications to increase its chemical stability and resistance to nucleases ( 36, 37 ), and it shows
The Protein‐Templated Synthesis of Enzyme‐Generated Aptamers
The resultant protein-templated EGA mixtures (T-EGA) and corresponding non-templated control mixtures (NT-EGA), where the aptamers are formed in the absence of the target demonstrated similar size profiles, when the ratio of the initiator and template are kept at a 1 : 1 ratio. These broad sized products result from the fact that each pre
PDB-101: Molecule of the Month: Fluorescent RNA Aptamers
RNA aptamers are being engineered to track molecules inside living cells. Spinach fluorescent aptamer, with RNA in light orange and fluorophore in green. Download high quality TIFF image. Scientists are constantly looking for new tools to explore cells in greater and greater detail. Green fluorescent protein is an example of a tool that opened
The isolation of an RNA aptamer targeting to p53 protein
However, an RNA aptamer that can distinguish a protein with a single amino acid mutation from the wild-type (WT) protein remains absent (3 ⇓ ⇓ ⇓ ⇓ ⇓ ⇓ ⇓ -11). Protein with a single amino acid substitution is the cause of a plethora of human diseases (12 ⇓ -14).
RNA aptamer-based sensitive detection of SARS coronavirus
Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection ofSARS-CoV N protein.
Characterization of Aptamer-Protein Complexes by X-ray
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms. While reports of new aptamers are numerous, characterization of their specific interaction is often restricted to the affinity of binding (KD). Over the years, crystal structures of aptamer-protein complexes have
Electrostatic surface potentials of protein-binding
The parameters of the aptamer-protein complexes display considerable variation. For instance, the interface area ranges from 410 [65] to 2088 Å 2 [36], while the binding affinities between each
Hybrid Aptamer‐Molecularly Imprinted Polymer (aptaMIP
The polymerizable aptamer was then incubated with the protein derivatized glass beads to form a protein-aptamer complex, with polymerization conducted around the immobilized complex. N -Isopropylacrylamide (NIPAm) was used as the major component in the polymer recipe, which enables thermoresponsive properties in the resultant NPs.
Detection of SARS-CoV-2 RNA Using a DNA Aptamer Mimic of
To detect RNA, we developed Lettuce, a fluorogenic DNA aptamer that binds and activates the fluorescence of DFHBI-1T, an otherwise nonfluorescent molecule that resembles the chromophore found in green fluorescent protein. Lettuce was selected from a randomized DNA library based on binding to DFHBI-agarose.
Atomic Scale Interactions between RNA and DNA Aptamers
Interest in the design and manufacture of RNA and DNA aptamers as apta-biosensors for the early diagnosis of blood infections and other inflammatory conditions has increased considerably in recent years. The practical utility of these aptamers depends on the detailed knowledge about the putative interactions with their target proteins. Therefore, understanding the aptamer-protein interactions
Identification and Affinity Determination of Protein
Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of prot
